A new and improved green fluorescent protein, named mNeonGreen, was developed.
It was engineered from a Yellow fluorescent protein (LanYFP) that was isolated from the cephalochordate Branchiostoma lanceolatum. Therefore, LanYFP is genetically unrelated to the commonly used Aequorea victoria GFP.
Directed evolution to make it a monomer produced the new, monomeric protein, mNeonGreen. The ex/em of mNeonGreen is slightly shifted to the yellow compared to EGFP (509/516 compared to 488/509), making it a better choice to separate from CFP emission.
Though slightly less brighter than its parent protein LanYFP, mNeonGFP is 2-3 times brighter than EGFP and actually brighter than most green & yellow proteins.
Another great advantage of this new protein is that is it fast folding – the authors claim…
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